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anti human il 4 mab  (Bio X Cell)


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    Bio X Cell anti human il 4 mab
    Anti Human Il 4 Mab, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+il+4+mab/InVivoMAb+anti-human+IL-4/pm41086975-69-67-71
    Average 93 stars, based on 14 article reviews
    anti human il 4 mab - by Bioz Stars, 2026-10
    93/100 stars

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    Cell Culture:

    Article Title: ALKBH3-AS1 drives SMAD3 stabilization via YTHDF2: Uncovering a pathogenic pathway for Th17 dysregulation in SLE.
    Article Snippet: Cells purified from mice were cultured in medium containing 5 μg/mL anti-mouse CD3 mAb (BE0001–1, BioXcell), 50 mM 2- mercaptoethanol (S6250, Sigma), 1 μg/mL anti-mouse CD28mAb (102102, BioLegend), 5 μg/mL anti-mouse IFN-g mAb (BE0045, BioXcell), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 20 ng/mL mouse IL-6 (216–16, PeproTech), and 5 μg/ mL anti-mouse IL-4 mAb (BE0055, BioXcell) for 3 days. .. Cells collected from humans were cultured in medium containing 50 mM 2-mercaptoethanol (Sigma), 5 μg/mL anti-human CD3 mAb (317349, BioLegend), 5 ng/mL human TGF-β (240-B-010, R&D Systems), 1 μg/mL soluble anti-human CD28 mAb (BE0291, BioXcell), 10 ng/mL human IL-23 (1290-IL-10, R&D Systems), 5 μg/mL anti-human IFN-g mAb (285-IF-100, R&D Systems), 20 ng/mL human IL-6 (206-IL-200, R&D Systems), 10 ng/mL human IL1β (201-LB-025, R&D Systems), and 5 μg/mL anti-human IL-4 mAb (BE0240, BioXcell) for 6–7 days. .. Human naïve CD4+ T cells were added to microplate wells in the presence or absence of ornithine (ORN, 100 nM, Sigma) and difluoromethylornithine (DFMO, 20 nM, Tocris) to identify molecules upstream of ALKBH3-AS1 during the differentiation of Th17 cells.



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    Ratio of Th1/Tc1 to Th2/Tc2 in peripheral blood of non-atopic and atopic patients with AA. ( a – d ) The expression of cytoplasmic cytokines was analyzed by flow cytometric analysis. The ratios of IFN–γ/IL-4 and IFN–γ/IL-13 in CD4 + ( a , b ) and CD8 + T cells ( c , d ) were measured in normal control subjects, non-atopic AA, and AA with extrinsic or intrinsic AA. ( e ) The correlation between the frequency of IFN–γ-producing cells and the serum IgE level was analyzed in the patients with non-atopic AA. ( f ) The ratio of CD8 + CXCR3 + (Tc1) to CD8 + CCR4 + (Tc2) cells was analyzed by flow cytometric analysis in normal control subjects, non-atopic AA, and AA with extrinsic or intrinsic AA.

    Journal: International Journal of Molecular Sciences

    Article Title: Immunological Properties of Atopic Dermatitis-Associated Alopecia Areata

    doi: 10.3390/ijms22052618

    Figure Lengend Snippet: Ratio of Th1/Tc1 to Th2/Tc2 in peripheral blood of non-atopic and atopic patients with AA. ( a – d ) The expression of cytoplasmic cytokines was analyzed by flow cytometric analysis. The ratios of IFN–γ/IL-4 and IFN–γ/IL-13 in CD4 + ( a , b ) and CD8 + T cells ( c , d ) were measured in normal control subjects, non-atopic AA, and AA with extrinsic or intrinsic AA. ( e ) The correlation between the frequency of IFN–γ-producing cells and the serum IgE level was analyzed in the patients with non-atopic AA. ( f ) The ratio of CD8 + CXCR3 + (Tc1) to CD8 + CCR4 + (Tc2) cells was analyzed by flow cytometric analysis in normal control subjects, non-atopic AA, and AA with extrinsic or intrinsic AA.

    Article Snippet: Then, intracellular cytokines were stained with FITC-conjugated mouse anti-human IFN-γ mAb (BD Biosciences), PE-conjugated anti-human IL-4 mAb (BD Biosciences), or PE-conjugated anti-human IL-13 mAb (BD Bioscience) for 30 min. After washing, stained cells were used for flow cytometric analysis on FACSCanto II.

    Techniques: Expressing